Table 5. Comparison of BGS activity of cell-free supernatant extracts by solvent

Sample	Volume (mL)	Specific activity (AU/mL)1)	Purification fold	Total activity (AU)2)	Recovery (%)
K748 supernatant	100	320	-	32,000	-
Hexane ext.	5	160	0.25	800	2.5
Ethyl acetate ext.	5	2,560	8	12,800	40
Butanol ext.	5	640	2	3,200	10

The activity per 1 mL sample is defined as 20 AU.
Volume (mL) * Specific activity (AU/mL).