Table 3. Enzyme assay for major biochemical reactions of Lactobacillus pentosus BMSE-K006 and K009 isolated from kefir using API ZYM kit

Enzyme Assayed For	K006	K009
Control	Colorless or color of the sample if it has an intense coloration
Alkaline phosphatase	-1)	-
Esterase (C4)	-	-
Esterase lipase (C8)	-	-
Lipase (C14)	-	-
Leucine arylamidase	+	+
Valine arylamidase	+	+
Crystine arylamidase	w	-
Trypsin	-	-
α-Chymotrypsin	-	-
Acid phospatase	w	w
Naphtol-AS-BI-phosphohydrolase	w	w
α-Galactosidase	w	w
β-Glucuronidase	+	+
β-Glucosidase	-	-
α-Glucosidase	+	+
β-Glucosidase	+	+
N-Acetyl-β-glucosaminidase	+	+
α-Mannosidase	-	-
α-Fucosidase	-	-

-; negative, +; positive, w; weak positive.